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IJC_25648_sm_suppfig-S1.tif32897KSupplementary Figure 1 CysLT1R and CysLT2R expression in breast cancer tissue expression. (a) Distribution of low and high staining of CysLT1R in small and large tumors, respectively. (b) Distribution of low and high staining of CysLT2R in small and large tumors, respectively.
IJC_25648_sm_suppfig-S2.tif21887KSupplementary Figure 2 CysLT2R signaling does not significantly affect cell proliferation and apoptosis. The effects of CysLT2R signaling on proliferation were estimated by cell counting at time points 0, 24, and 48 hours in (a) MDA-MB-231 and (b) MCF-7 cells. Cells were either left non-transfected (black bars), or transfected with empty vector (light grey bars) or with Flag-CysLT2R vector (dark grey bars). These cells were then grown in the absence or presence of 40 nM LTC4 for 24 or 48 hours before being counted and expressed as a percent of the number of cells present at 0 hour. (c) MCF-7 cells were treated as in panel (b) but analyzed for number of apoptotic cells by staining with Trypan Blue. The percentage of apoptotic cells was determined after incubation of the cells in the absence or presence of 40 nM LTC4 and tamoxifen for 24 hours. In the diagram, the results of at least three separate experiments are presented and given as means ± SE; *, P < 0.05.

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