Smooth muscle actin-expressing stromal fibroblasts in head and neck squamous cell carcinoma: Increased expression of galectin-1 and induction of poor prognosis factors

Authors

  • Jaroslav Valach,

    1. Department of Stomatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
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    • J.V. and Z.F. contributed equally to this work

  • Zdeněk Fík,

    1. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University, Prague, Czech Republic
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    • J.V. and Z.F. contributed equally to this work

  • Hynek Strnad,

    1. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Martin Chovanec,

    1. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University, Prague, Czech Republic
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  • Jan Plzák,

    1. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University, Prague, Czech Republic
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  • Zdeněk Čada,

    1. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University, Prague, Czech Republic
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  • Pavol Szabo,

    1. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Jana Šáchová,

    1. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Miluše Hroudová,

    1. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Markéta Urbanová,

    1. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Martin Šteffl,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Jan Pačes,

    1. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Jiří Mazánek,

    1. Department of Stomatology, First Faculty of Medicine, Charles University, Prague, Czech Republic
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  • Čestmír Vlček,

    1. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
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  • Jan Betka,

    1. Department of Otorhinolaryngology, Head and Neck Surgery, First Faculty of Medicine, Charles University, Prague, Czech Republic
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  • Herbert Kaltner,

    1. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany
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  • Sabine André,

    1. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany
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  • Hans-Joachim Gabius,

    1. Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany
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  • Roman Kodet,

    1. Department of Pathology and Molecular Medicine, Second Faculty of Medicine, Charles University, Prague, Czech Republic
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  • Karel Smetana Jr,

    Corresponding author
    1. Institute of Anatomy, First Faculty of Medicine, Charles University, Prague, Czech Republic
    • Charles University, First Faculty of Medicine, Institute of Anatomy, U nemocnice 3, 128 00 Prague 2, Czech Republic
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  • Peter Gál,

    1. Institute for Histology and Embryology, First Faculty of Medicine, Charles University, Prague, Czech Republic
    2. Department for Biomedical Research, East-Slovak Institute of Cardiovascular Diseases, Košice, Slovak Republic
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  • Michal Kolář

    Corresponding author
    1. Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    • Academy of Sciences of the Czech Republic, Institute of Molecular Genetics, Laboratory of Genomics and Bioinformatics, Vídeňská 1083, 142 20 Prague 4, Czech Republic
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Abstract

Tumor stroma is an active part influencing the biological properties of malignancies via molecular cross-talk. Cancer-associated fibroblasts play a significant role in this interaction. These cells frequently express smooth muscle actin and can be classified as myofibroblasts. The adhesion/growth-regulatory lectin galectin-1 is an effector for their generation. In our study, we set the presence of smooth muscle actin-positive cancer-associated fibroblasts in relation to this endogenous lectin and an in vivo competitor (galectin-3). In squamous cell carcinomas of head and neck, upregulation of galectin-1 presence was highly significantly correlated to presence of smooth muscle actin-positive cancer-associated fibroblasts in the tumor (p = 4 × 10−8). To pinpoint further correlations on the molecular level, we applied microarray analyses to the transcription profiles of the corresponding tumors. Significant correlations of several transcripts were detected with the protein level of galectin-1 in the cancer-associated fibroblasts. These activated genes (MAP3K2, TRIM23, PTPLAD1, FUSIP1, SLC25A40 and SPIN1) are related to known squamous-cell-carcinoma poor-prognosis factors, NF-κB upregulation and splicing downregulation. These results provide new insights into the significance of presence of myofibroblasts in squamous cell carcinoma.

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