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IJC_27572_sm_SuppFig1.tif1521KSupporting Figure 1. TGF-β neutralization decreased tumor recurrences in mice received 1D11 compared to 13C4 treatment. Tumor bearing mice were treated with 1D11 (n=22( or 13C4 (n=22). The tumors were surgically removed on day 14. The mice with tumor recurrence were recorded on day 42.
IJC_27572_sm_SuppFig2.tif1521KSupporting Figure 1. No difference of Gr-1+CD11b+ cell production between 1D11 and 13C4 treatment was observed in bone marrow (A) or spleen (B) of tumor bearing mice. The Gr-1+CD11b+ cells were analyzed by flow cytometry (n=3(. Bar shows median ± SEM.
IJC_27572_sm_SuppFig3.tif1521KSupporting Figure 1. No difference in Gr-1+CD11b+ cell apoptosis between 1D11 and 13C4 treatment was observed in blood, spleen and LNs of 4T1 tumor-bearing mice. Cells from peripheral blood, spleen, and LN of mice bearing 4T1 tumor were stained with Gr-1, CD11b, Annexin V and 7-AAD. The percentage of 7-AAD and Annexin positive cells within Gr-1+CD11b+ cell population was analyzed by flow cytometry. The percentage of 7-AAD and Annexin positive cells within Gr-1+CD11b+ cell population was analyzed by flow cytometry. Representative dot plots (A) and statistic results (B) are shown. Error bar = SEM. One of three independent experiments is shown.
IJC_27572_sm_SuppFig4.tif1521KSupporting Figure 4. TGF-β neutralization decreased lung metastasis in mice with peripheral Gr-1+CD11b+ cells in 60-90% but not those with peripheral Gr-1+CD11b+ cells in 10-60%. A large cohort of tumor bearing mice were given 1D11 (n=20) or 13C4 (n=20) treatment, on day 12, the tumor was surgically removed. Peripheral Gr-1+CD11b+ cell percentage and Lung metastasis were evaluated on Day 42.

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