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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
IJC_27682_sm_SuppFig1.tif7437KSupporting Information Figure 1. The restriction molecule and the minimal epitope in recognition of the NY-ESO-1f peptide by the CD8 T-cell clone 2H10. The CD8 T-cell clone 2H10 (5 × 103) obtained from the culture shown in Table1 was cultured with an equal number of irradiated (100 Gy), autologous EBV-B cells pulsed with the NY-ESO-1f peptide (1 μM). The restriction molecule was analyzed by antibody blocking (5 μg/ml) (A) and using various EBV-B cells as APC (B). The minimal epitope was determined by using various N- and C-termini truncated peptides (1 μM) (C) and by titration of the peptide concentration (D). In E, the binding of the NY-ESO-1 92-100/B*35:01 and NY-ESO-1 92-102/B*35:01 tetramers to the clone 2H10 is shown. NY-ESO-1 91-101/A*24:02, NY-ESO-1 94-104/B*35:01 and HIV Env/A*24:02 tetramers were used as controls.
IJC_27682_sm_SuppFig2.tif7265KSupporting Information Figure 2. The restriction molecule and the minimal epitope in recognition of the NY-ESO-1f peptide by the CD8 T-cell clone 8D5. The CD8 T-cell clone 8D5 (5 × 103) obtained from the culture shown in Table1 was cultured with an equal number of irradiated (100 Gy), autologous EBV-B cells pulsed with the NY-ESO-1f peptide (1 μM). The restriction molecule was analyzed by antibody blocking (5 μg/ml) (A) and using various EBV-B cells as APC (B). The minimal epitope was determined by using various N- and C-termini truncated peptides (1 μM) (C) and by titration of the peptide concentration (D). In E, the binding of the NY-ESO-1 94-104/B*35:01 tetramer to the clone 8D5 is shown. NY-ESO-1 91-101/A*24:02, NY-ESO-1 92-100/B*35:01, NY-ESO-1 92-102/B*35:01 and HIV Env/A*24:02 tetramers were used as controls.
IJC_27682_sm_SuppFig3.tif6102KSupporting Information Figure 3. The restriction molecule and the minimal epitope in recognition of the NY-ESO-1f peptide by the CD8 T-cell clone 10-10U. The CD8 T-cell clone 10-10U (5 × 103) obtained from the culture shown in Table1 was cultured with an equal number of irradiated (100 Gy), autologous EBV-B cells pulsed with the NY-ESO-1f peptide (1 μM). The restriction molecule was analyzed by antibody blocking (5 μg/ml) (A) and using various EBV-B cells as APC (B). The minimal epitope was determined by using various N- and C-termini truncated peptides (1 μM) (C) and by titration of the peptide concentration (D).
IJC_27682_sm_SuppFig4.tif7394KSupporting Information Figure 4. The restriction molecule and the minimal epitope in recognition of the NY-ESO-1f peptide by the CD8 T-cell clone 7B. The CD8 T-cell clone 7B (5 × 103) obtained from the culture shown in Table1 was cultured with an equal number of irradiated (100 Gy), autologous EBV-B cells pulsed with the NY-ESO-1f peptide (1 μM). The restriction molecule was analyzed by antibody blocking (5 μg/ml) (A) and using various EBV-B cells as APC (B). The minimal epitope was determined by using various N- and C-termini truncated peptides (1 μM) (C) and by titration of the peptide concentration (D). In E, the binding of the NY-ESO-1 96-106/C*12:02 tetramer to clone 7B is shown. NY-ESO-1 91-101/A*24:02, NY-ESO-1 92-102/B*35:01, NY-ESO-1 94-104/B*35:01 and HIV Env/A*24:02 tetramers were used as controls.

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