SEARCH

SEARCH BY CITATION

Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
IJC_27724_sm_SuppFig1.tif28042KSupporting Information Figure S1. A. Plumbagin inhibits Glut-1 protein expressions in PEO-4 and OVCAR-5 cells. PEO-4 and OVCAR-5 cells were treated with increasing concentrations of plumbagin and were kept in normoxic and hypoxic conditions for 12 hr. Plumbagin inhibited expressions of Glut-1 in both normoxia and hypoxia. β-Actin was used as a loading control. B. Effect of plumbagin on HIF-2α, and MAPK-42/44 phosphorylation in OVCAR-5 cells. OVCAR-5 cells were treated with 1, 5 or 10 μM doses of plumbagin and were kept in normoxic and hypoxic conditions for 12 hr. Plumbagin inhibited the HIF-2α expression, in normoxia as well as in hypoxia. MAPK-42/44 phosphorylation was strongly induced by the compound in both normoxia and hypoxia. C. Plumbagin induces basal level of Ca2+ through ROS. Serum starved HUVEC cells were pre-treated with NAC (10 mM) for 1.5 hr and then media was changed and treated with DMSO or 500 nM/1 μM of plumbagin for 12 hr and then used for intracellular Ca2+ mobilization assay. D. Effect of plumbagin on intracellular Ca2+ release. Serum starved HUVEC cells were co-treated with BAPTA-AM (10 μM), and DMSO or 500 nM/1 μM of plumbagin for 12 hr and then used for intracellular Ca2+ mobilization assay.

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.