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Additional Supporting Information may be found in the online version of this article.

FilenameFormatSizeDescription
IJC_27812_sm_SuppFigS1.tif142KSupporting Information Figure 1. Positive and negative controls for Figure 2A. (A) SHH:SW480 (positive), CT26 (negative); (B) SMO: human heat lysate (positive), human heart lysate with immunizing peptide (negative); (C) GLI1:HT29 (positive), CT26 (negative); (D) PTCH1:HT29 (positive), HCT116 (negative)
IJC_27812_sm_SuppFigS2.tif51KSupporting Information Figure 2. PTCH2 and HHIP mRNA levels are not correlated. The mRNA levels of (A) PTCH2 and (B) HHIP were detected by qRT-PCR. GAPDH was used as a internal control.
IJC_27812_sm_SuppFigS3A.tif249KSupporting Information Figure 3A. Methylation analysis of PTCH1 promoter in n-ACF (A), d-ACF (B), and adjacent normal mucosa. Methylation-specific PCR using primers for methylated (M) and unmethylated (U) alleles of bisulfate-treated DNA was performed. Loci examined and ACF numbers were indicated above each gel. MA: marker, N: normal mucosa;
IJC_27812_sm_SuppFigS3B.tif134KSupporting Information Figure 3B. Methylation analysis of PTCH1 promoter in n-ACF (A), d-ACF (B), and adjacent normal mucosa. Methylation-specific PCR using primers for methylated (M) and unmethylated (U) alleles of bisulfate-treated DNA was performed. Loci examined and ACF numbers were indicated above each gel. MA: marker, N: normal mucosa;
IJC_27812_sm_SuppTabS1.doc112KSupporting Information Table 1.
IJC_27812_sm_SuppTabS2.doc30KSupporting Information Table 2.

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