Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen

Authors

  • J. Bubeník,

    1. Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia; Laboratory of Oncology, Faculty of General Medicine, Charles University, Prague; and Radiological Clinic, Faculty of General Medicine, Charles University, Prague, Czechoslovakia
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  • M. Barešová,

    1. Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia; Laboratory of Oncology, Faculty of General Medicine, Charles University, Prague; and Radiological Clinic, Faculty of General Medicine, Charles University, Prague, Czechoslovakia
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  • V. Viklický,

    1. Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia; Laboratory of Oncology, Faculty of General Medicine, Charles University, Prague; and Radiological Clinic, Faculty of General Medicine, Charles University, Prague, Czechoslovakia
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  • J. Jakoubková,

    1. Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia; Laboratory of Oncology, Faculty of General Medicine, Charles University, Prague; and Radiological Clinic, Faculty of General Medicine, Charles University, Prague, Czechoslovakia
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  • H. Sainerová,

    1. Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia; Laboratory of Oncology, Faculty of General Medicine, Charles University, Prague; and Radiological Clinic, Faculty of General Medicine, Charles University, Prague, Czechoslovakia
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  • J. Donner

    1. Institute of Experimental Biology and Genetics, Czechoslovak Academy of Sciences, Prague 6, Czechoslovakia; Laboratory of Oncology, Faculty of General Medicine, Charles University, Prague; and Radiological Clinic, Faculty of General Medicine, Charles University, Prague, Czechoslovakia
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Abstract

A cell line derived from human urinary bladder carcinoma, designated T24, was established in vitro. The growth of T24 cells in tissue culture was characterized by a disorderly pattern of growth in one or more layers and by mixed epithelioid-fibroblastoid morphology. The generation time of T24 cells was 19 h. The cells had a hypotetraploid stemline with marker chromosomes. The malignant character of the line was verified by inoculation of cell suspension into hamster cheek pouch. The presence of tumour-specific antigen (TSA) in T24 cells was demonstrated by a micromodification of the cytotoxicity test with autochthonous leukocytes and serum from the donor of the tumour. The TSA was also well detectable by the reaction with allogeneic leukocytes from tumour-bearing patients and persisted during a 30-month culture period. The permanent presence of TSA in this carcinoma cell line suggests that the TSA is a genetically determined characteristic of T24 cells.

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