Characterization of a human papillomavirus from epidermodysplasia verruciformis lesions of a patient from upper-volta

Authors

  • Herbert Pfister,

    1. Institut für Virologie, Zentrum für Hygiene, Hermann-Herder-Strasse 11, 7800 Freiburg, West Germany
    2. Hautklinik und Poliklinik der Freien Universität Berlin im Rudolf-Virchow-Krankenhaus, Augustenburgerplatz 1, 1000 Berlin 65
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  • Friedrich Nürnberger,

    1. Institut für Virologie, Zentrum für Hygiene, Hermann-Herder-Strasse 11, 7800 Freiburg, West Germany
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  • Lutz Gissmann,

    1. Institut für Virologie, Zentrum für Hygiene, Hermann-Herder-Strasse 11, 7800 Freiburg, West Germany
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  • Harald Zur Hausen

    1. Institut für Virologie, Zentrum für Hygiene, Hermann-Herder-Strasse 11, 7800 Freiburg, West Germany
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Abstract

A case of epidermodysplasia verruciformis in a patient from Upper-Volta is described. Slightly elevated, flat warts were observed on hands, feet, arms and legs, and pityriasis versicolor-like lesions were found mainly on the trunk. The patient showed no malignant tumors. Histological examination revealed hyperkeratosis, granulosis and moderate acanthosis with large, foamy, basophilic keratinocytes in stratum granulosum and stratum spinosum. Papillomavirus particles could be prepared from these lesions and were differentiated from known papillomavirus types by immune electron microscopy with monospecific antisera and by DNA-DNA hybridization. The viral DNA was characterized by cleavage with several restriction endonucleases and a physical map of the resulting fragments was established. The virus is designated as HPV 8. Preliminary seroepidemiologic studies with human sera indicate a rather wide distribution of HPV 8. Blot hybridization of DNA from human carcinomas with 32P-labelled virus DNA detected no HPV 8-specific sequences.

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