A cell-surface antigen which is present in the ganglioside fraction and shared by human melanomas

Authors

  • M.-Y. Yeh,

    1. Programs in Tumor Immunology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    2. Programs in Departments of Microbiology/Immunology, University of Washington, Seattle, WA 98195, USA.
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  • I. Hellström,

    Corresponding author
    1. Programs in Tumor Immunology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    2. Programs in Departments of Microbiology/Immunology, University of Washington, Seattle, WA 98195, USA.
    Current affiliation:
    1. Dept. of Microbiology and Immunology, National Defence Medical Center, P.O Box 8244, Taipei, Taiwan, Republic of China
    • Fred Hutchinson Cancer Research Center, 1124 Columbia Street, Seattle, Washington 98104
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  • K. Abe,

    1. Programs in Biochemical Oncology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    2. Programs in Pathobiology, University of Washington, Seattle, WA 98195, USA.
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  • S. Hakomori,

    1. Programs in Biochemical Oncology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    2. Programs in Pathobiology, University of Washington, Seattle, WA 98195, USA.
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  • K. E. Hellström

    1. Programs in Tumor Immunology, Fred Hutchinson Cancer Research Center, Seattle, WA 98104
    2. Programs in Pathology, University of Washington, Seattle, WA 98195, USA.
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Abstract

We have raised a hybridoma, 4.2, which forms an IgM antibody to an antigen expressed on cultivated cells from most human melanomas, more infrequently on cells from other human tumors, and very weakly on cultivated fibroblasts, T cells and B cells. The antigen can be detected in biopsy material of human melanoma and, in smaller amounts, in fresh samples of normal human lung and kidney. Preliminary biochemical characterization indicates that it is associated with the ganglioside fraction. We feel that the antigen (referred to as 4.2) has a sufficient degree of tumor specificity to be rated as a human melanoma-associated antigen.

Ancillary