Human papillomavirus infection and anal cancer

Authors

  • Anna Marie Beckmann,

    Corresponding author
    1. Fred Hutchinson Cancer Research Center, Division of Clinical Research, Seattle, WA 98104
    2. Department of Pathology, School of Medicine, University of Washington, Seattle, WA 98195
    • Dept. of Experimental Pathology, Fred Hutchinson Cancer Research Center, 1124 Columbia Street. Seattle, WA 98104
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  • Janet R. Daling,

    1. Fred Hutchinson Cancer Research Center, Division of Public Health Sciences, Seattle, WA 98104
    2. Department of Epidemiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
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  • Karen J. Sherman,

    1. Fred Hutchinson Cancer Research Center, Division of Public Health Sciences, Seattle, WA 98104
    2. Department of Epidemiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
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  • Christopher Maden,

    1. Fred Hutchinson Cancer Research Center, Division of Public Health Sciences, Seattle, WA 98104
    2. Department of Epidemiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
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  • Beth A. Miller,

    1. Fred Hutchinson Cancer Research Center, Division of Clinical Research, Seattle, WA 98104
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  • Ralph J. Coates,

    1. Fred Hutchinson Cancer Research Center, Division of Public Health Sciences, Seattle, WA 98104
    Current affiliation:
    1. Department of Epidemiology and Biostatistics, Emory University, Atlanta, GA 30322
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  • Nancy B. Kiviat,

    1. Department of Pathology, School of Medicine, University of Washington, Seattle, WA 98195
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  • David Myerson,

    1. Fred Hutchinson Cancer Research Center, Division of Clinical Research, Seattle, WA 98104
    2. Department of Pathology, School of Medicine, University of Washington, Seattle, WA 98195
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  • Noel S. Weiss,

    1. Fred Hutchinson Cancer Research Center, Division of Public Health Sciences, Seattle, WA 98104
    2. Department of Epidemiology, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195, USA
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  • T. Gregory Hislop,

    1. Cancer Control Agency of British Columbia, Vancouver, B.C., V52 4EG Canada
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  • Marjorie Beagrie,

    1. Cancer Control Agency of British Columbia, Vancouver, B.C., V52 4EG Canada
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  • James K. McDougall

    1. Fred Hutchinson Cancer Research Center, Division of Clinical Research, Seattle, WA 98104
    2. Department of Pathology, School of Medicine, University of Washington, Seattle, WA 98195
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Abstract

To study the association of human papillomavirus (HPV) infection with anal cancer, we examined tissue specimens from 126 patients with malignant lesions of the anal skin or mucosa. The patients were enrolled in a population-based, case-control study of ano-rectal cancer which is being conducted in the state of Washington and the Province of British Columbia. Histologic sections from formalin-fixed, paraffinembedded tissues were tested for the presence of HPV DNA by in situ hybridization with biotin-labelled HPV 6, 11, 16, 18 and 31 DNA probes. HPV DNA sequences were found in tumor tissues from 24 of the 126 subjects (19.0%). When only squamous neoplasms are considered, 23 of 70 subjects (32.9%) had lesions which contained detectable HPV DNA. One HPV-positive patient had a cloacogenic carcinoma that contained regions of squamous differentiation and it was in these squamous cells that HPV DNA was localized. Of the 23 squamous lesions that harbored detectable HPV DNA, 8 contained HPV 6, 10 contained HPV 16, I contained HPV 18 and 4 contained an unclassified virus type(s). HPV DNA was found in tissues from 14 patients with carcinoma-in situ and 10 subjects with invasive carcinoma. These results demonstrate that some malignant tumors of the anus, in both men and women, are associated with HPV infection. We conclude that the anal squamous epithelium is another site where infection with the common genital tract HPVs may carry a risk of malignant transformation.

Ancillary