Fetal myofibroblast-like cells isolated from post-radiation fibrosis in human breast cancer
Article first published online: 18 JUL 2006
Copyright © 1991 Wiley-Liss, Inc., A Wiley Company
International Journal of Cancer
Volume 47, Issue 5, pages 697–702, 12 March 1991
How to Cite
Brouty-Boyé, D., Raux, H., Azzarone, B., Tamboise, A., Tamboise, E., BéRanger, S., Magnien, V., Pihan, I., Zardi, L. and Israël, L. (1991), Fetal myofibroblast-like cells isolated from post-radiation fibrosis in human breast cancer. Int. J. Cancer, 47: 697–702. doi: 10.1002/ijc.2910470512
- Issue published online: 18 JUL 2006
- Article first published online: 18 JUL 2006
- Manuscript Revised: 15 NOV 1990
- Manuscript Received: 13 SEP 1990
- Association pour la Recherche sur le Cancer, Villejuif, France.
Cells were isolated from post-radiation fibrosis biopsies of patients with recurrent breast carcinoma. These cells were identified as fibroblasts and compared with fibroblasts from normal breast tissues for their proliferative activities, chromosome number and for the presence of various components of the extracellular matrix and cytoskeleton. The proliferative activity of the fibrosis-derived fibroblasts did not significantly differ from that of normal breast fibroblasts. Both cell types required serum to grow and did not form colonies in soft agar. Cells from 2 of the 3 fibroses analyzed displayed aneuploid karyotypes with multiple structural abnormalities. All of the fibroblastic cells produced types I, III and V collagen, fibronectin and vimentin. However, in contrast to normal breast fibroblasts, fibrosis-derived cells produced high amounts of oncofetal fibronectin. In addition, fibrosis fibroblasts also expressed the a-actin isoform which is specific for smooth-muscle cells. These results suggest that post-radiation fibrosis in malignant breast contains atypical fibroblasts with fetal and myofibroblastic characteristics.