Aberrations of tumor-suppressor genes (p53, apc, mcc and Rb) in esophageal squamous-cell carcinoma

Authors

  • Chihaya Maesawa,

    Corresponding author
    1. Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
    2. Department of Pathophysiology, Division of Clinical Research, Morioka National Hospital, Aoyama 1-25-1, 020 Morioka, Japan
    • Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
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  • Gen Tamura,

    1. Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
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  • Yasushi Suzuki,

    1. Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
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  • Satoshi Ogasawara,

    1. Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
    2. Department of Surgery, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
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  • Kaoru Ishida,

    1. Department of Surgery, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
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  • Kazuyoshi Saito,

    1. Department of Surgery, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
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  • Ryoichi Satodate

    1. Department of Pathology, Iwate Medical University School of Medicine, Uchimaru 19-1, Japan
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Abstract

Loss of heterozygosity at 4 tumor-suppressor gene loci (p53, apc, mcc and Rb) was investigated using polymerase chain reactions, in 49 esophageal squamous-cell carcinoma specimens from patients who had undergone curative resection. Mutations in the p53 gene within exons 5 to 8 were also examined. LOH was detected in 9 (43%) of 21 p53 genes, 16 (55%) of 29 apc genes, 10 (48%) of 21 mcc genes, and 13 (52%) of 25 Rb genes for which heterozygosity could be determined. Mutations in the p53 gene were detected in 18 (36%) of 49 cases and were significantly more frequent in stage-III tumors and in tumors exhibiting DNA aneuploidy. In 5 cases where heterozygosity could be determined for all the loci, all had 2 or more aberrations. Additionally, a heterozygous deletion of p53 gene was associated with a mutation of the remaining allele in 8 (89%) of 9 cases. Short-term relapse within 3 to 12 months occurred significantly more frequently in patients having tumors with both p53 aberrations (p < 0.05). Thus, aberration of tumor-suppressor genes was a frequent occurrence in esophageal squamous-cell carcinoma and inactivation of the p53 gene may contribute to the progression of this tumor. © Wiley-Liss, Inc.

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