The chondroitin sulfate proteoglycan NG2 is a tumour-specific antigen on the chemically induced rat chondrosarcoma HSN

Authors

  • Olivier Léger,

    1. Section of immunology. Institute of Cancer Research, Belmont, Sutton, Surrey, SM2 5NG, UK
    Current affiliation:
    1. MRC Collaborative Centre, Antibody Engineering Group, 1-3 Burtonhole Lane, Mill Hill, London, NW7 1AD, UK
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  • Caroline Johnson-Léger,

    1. Section of immunology. Institute of Cancer Research, Belmont, Sutton, Surrey, SM2 5NG, UK
    Current affiliation:
    1. National Institute for Medical Research, Cellular Immunology, The Ridgeway, Mill Hill, London, NW7 1AA, UK
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  • Elizabeth Jackson,

    1. Section of immunology. Institute of Cancer Research, Belmont, Sutton, Surrey, SM2 5NG, UK
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  • Brian Coles,

    1. CRC Molecular Toxicology' Group, Department of Biochemistry, University College and Middlesex School of Medicine, Windeyer Building, Cleveland Street, London, W1P 6DB, UK
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  • Christopher Dean

    Corresponding author
    1. Section of immunology. Institute of Cancer Research, Belmont, Sutton, Surrey, SM2 5NG, UK
    • Section of Immunology, Institute of Cancer Research, Haddow Laboratories, 15 Cotswold Road, Belmont, Sutton, Surrey, SM2 5NG, UK. Fax: 081 643 0223
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Abstract

N-terminal sequences of 19 and 11 amino acids obtained from 2 different Cryptic fragments of the tumour-specific antigen on the chemically induced rat chondrosarcoma HSN show a 100% homology with the rat chondroitin sulfate proteoglycan NG2. Using a scheme of overlapping oligonucleotide primers we have cloned by PCR amplification the cDNA for the specific antigen of the HSN tumour that is immunogenic in immunocompetent CBH/Cbi rats and now report that its cDNA sequence is identical to that of NG2. The cDNA codes for a transmembrane protein of 2,325 amino acids with a large extracellular domain of 2,224 amino acids containing 2 cysteine-rich regions, a trans-membrane domain (25 amino acids) and a short cytoplasmic tail (76 amino acids). The tumour-specific determinant was found to lie between amino acid residues 556 and 992 on the core glycoprotein.

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