Retinoic acid and methotrexate specifically increase PHA-E-lectin binding to a 67-kDa glycoprotein in LA-N-1 human neuroblastoma cells

Authors

  • Sharon A. Ross,

    1. Cancer Prevention Fellowship Program, National Caner Institute, Bethesda, MD 20892, USA
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  • Carol S. Jones,

    1. Laboratory of Cellular Carcinogenesis and Tumor Promotion, Bethesda, MD 20892, USA
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  • Luigi M. De Luca

    Corresponding author
    1. Laboratory of Cellular Carcinogenesis and Tumor Promotion, Bethesda, MD 20892, USA
    • Laboratory of Cellular Carcinogenesis and Tumor Promotion, National Cancer Institute, Bldg. 37, 37, Rm. 3A17, 37 Convent Drive MSC 4255, Bethesda, MD 20892-4255, USA
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Abstract

Retinoic acid (RA) decreased growth and increased morphologic differentiation of human neuroblastoma LA-N-1 cells. These phenomena correlated with a specific enhancement of PHA-E lectin binging to a glycoprotein of MW 67 kDa (gp67). Gp67 was found susceptible to N-glycanase and displayed BSA binding by affinity chromatography analysis. The chemotherapeutic agent methotrexate (MTX) also reduced growth and induce differentiation of LA-N-1 cells. In addition, the cells responded to MTX as well as to doxorubicin by a marked increase in PHA-E binding to gp67. We conclude that reduced growth and induction of morphological differentiation of LA-N-1 cells correlated with increased binding of PHA-E to gp67. © 1995 Wiley-Liss Inc.

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