De novo-generated small palindromes are characteristic of amplicon boundary junction of double minutes (pages 797–806)
Jing Zhu, Yang Yu, Xiangning Meng, Yihui Fan, Yu Zhang, Chunshui Zhou, Zhichao Yue, Yan Jin, Chunyu Zhang, Lisa Yu, Wei Ji, Xueyuan Jia, Rongwei Guan, Jie Wu, Jingcui Yu, Jing Bai, Xin-Yuan Guan, Mingrong Wang, Ki-Young Lee, Wenjing Sun and Songbin Fu
Version of Record online: 9 MAR 2013 | DOI: 10.1002/ijc.28084
Double minutes (DMs) are poorly understood hallmarks of gene amplification, a form of genomic aberration associated with tumor development. After cloning DMs from a human ovarian cancer cell line, the authors determined the precise breakpoints of two co-amplified amplicons and analyzed the complex DNA sequence joining them. They found that de novo creation of small palindromic sequences surrounding the breakpoints is a common characteristic of amplicon junction sequences in cancers. The palindromic sequences, which may be generated through non-homologous end joining in concert with a novel DNA repair machinery, may play a common role in amplicon rejoining and gene amplification.