This study empirically examined the relationship between the Big Five personality traits and subjective well-being (SWB) in India. SWB variables used were life satisfaction, positive affect and negative affect. A total of 183 participants in the age range 30–40 years from Pune, India, completed the personality and SWB measures. Backward stepwise regression analysis showed that the Big Five traits accounted for 17% of the variance in life satisfaction, 35% variance in positive affect and 28% variance in negative affect. Conscientiousness emerged as the strongest predictor of life satisfaction. In line with the earlier research findings, neuroticism and extraversion were found to predict negative affect and positive affect, respectively. Neither openness to experience nor agreeableness contributed to SWB. The research emphasises the need to revisit the association between personality and SWB across different cultures, especially non-western cultures.