MicroRNA-181b targets cAMP responsive element binding protein 1 in gastric adenocarcinomas

Authors

  • Lin Chen,

    1. Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China
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      The authors are contributed equally to this work.

  • Qian Yang,

    1. Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China
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      The authors are contributed equally to this work.

  • Wei-Qing Kong,

    1. Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China
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      The authors are contributed equally to this work.

  • Tao Liu,

    1. Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China
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  • Min Liu,

    1. Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China
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  • Xin Li,

    1. Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China
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  • Hua Tang

    Corresponding author
    1. Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, Tianjin, China
    • Tianjin Life Science Research Center and Basic Medical School, Tianjin Medical University, 22 Qi-Xiang-Tai Road, Tianjin 300070, China
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    • Tel./Fax: +86 22 23542503.


Abstract

MicroRNAs are a class of small endogenous non-coding RNAs that function as post-transcriptional regulators. In our previous study, we found that miR-181b was significantly downregulated in human gastric adenocarcinoma tissue samples compared to the adjacent normal gastric tissues. In this study, we confirm the down-regulation of miR-181b in human gastric cancer cell lines versus the gastric epithelial cells. Overexpression of miR-181b suppressed the proliferation and colony formation rate of gastric cancer cells. miR-181b downregulated the expression of cAMP responsive element binding protein 1 (CREB1) by binding its 3′ untranslated region. Overexpression of CREB1 counteracted the suppression of growth in gastric cancer cells caused by ectopic expression of miR-181b. These results indicate that miR-181b may function as a tumor suppressor in gastric adenocarcinoma cells through negative regulation of CREB1. © 2012 IUBMB IUBMB Life, 64(7): 628–635, 2012

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