Alantolactone inhibits growth of K562/adriamycin cells by downregulating Bcr/Abl and P-glycoprotein expression

Authors

  • Chunhui Yang,

    1. Department of Laboratory Hematology, College of Laboratory Medicine, Dalian Medical University, Dalian, People's Republic of China
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  • Jingbo Yang,

    1. Key Laboratory for Molecular and Chemical Genetics of Critical Human Diseases of Jilin Province, Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun, People's Republic of China
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  • Meiyan Sun,

    1. Key Laboratory for Molecular and Chemical Genetics of Critical Human Diseases of Jilin Province, Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun, People's Republic of China
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  • Jiangzhou Yan,

    1. Department of Laboratory Hematology, College of Laboratory Medicine, Dalian Medical University, Dalian, People's Republic of China
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  • Xiuxiang Meng,

    Corresponding author
    1. Department of Laboratory Hematology, College of Laboratory Medicine, Dalian Medical University, Dalian, People's Republic of China
    • Department of Laboratory Hematology, College of Laboratory Medicine, Dalian Medical University, Dalian 116044, People's Republic of China
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    • Tel.:+ +086-0411-86110389. Fax:+86-0411-86110392

  • Tonghui Ma

    1. Key Laboratory for Molecular and Chemical Genetics of Critical Human Diseases of Jilin Province, Central Research Laboratory, Jilin University Bethune Second Hospital, Changchun, People's Republic of China
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Abstract

Alantolactone, a sesquiterpene lactone containing an α-methylene-γ-lactone group, is the active component of Inula helenium (Compositae), a traditional Chinese medicinal herb. It has been reported that alantolactone has the capacity to inhibit tumor cell growth through induction of apoptosis. The purpose of this study was to assess the effects of alantolactone in the adriamycin (ADR)-resistant human erythroleukemia cell line K562/ADR, and provide evidence that it might function as a potent therapeutic agent in chronic myelogenous leukemia (CML) patients with Bcr/Abl and the multidrug-resistance phenotype. Our results showed that alantolactone significantly inhibited K562/ADR cell growth by downregulating Bcr/Abl and P-glycoprotein expression. Alantolactone also induced apoptosis via modulation of protein levels of Bcl-2 family members, caspase activation, poly ADP ribose polymerase cleavage, and cytochrome C release. We also observed that alantolactone induced cell-cycle arrest in the G2/M phase, downregulated cyclin B1 and cyclin-dependent protein kinase 1, and upregulated the cyclin-dependent kinase inhibitor p21. Together, these results demonstrate that alantolactone may be a potent therapeutic agent against CML, and a potential Bcr/Abl inhibitor. © 2013 IUBMB Life., 65(5):435–444, 2013.

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