The phytotoxin fusicoccin differently regulates 14-3-3 proteins association to mode III targets

Authors

  • Alessandro Paiardini,

    1. Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
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  • Patrizia Aducci,

    Corresponding author
    1. Department of Biology, University of Rome “Tor Vergata”, Rome, Italy
    • Address correspondence to: Patrizia Aducci, Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy. or Lorenzo Camoni, Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy; Tel., +39 06 72594343; Fax, +39 06 2023500.E-mail: aducci@uniroma2.it or E-mail: camoni@uniroma2.it.

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  • Laura Cervoni,

    1. Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
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  • Francesca Cutruzzolà,

    1. Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
    2. Istituto Pasteur-Fondazione Cenci Bolognetti, Rome, Italy
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  • Cristina Di Lucente,

    1. Department of Biology, University of Rome “Tor Vergata”, Rome, Italy
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  • Giacomo Janson,

    1. Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
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  • Stefano Pascarella,

    1. Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
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  • Serena Rinaldo,

    1. Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
    2. Istituto Pasteur-Fondazione Cenci Bolognetti, Rome, Italy
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  • Sabina Visconti,

    1. Department of Biology, University of Rome “Tor Vergata”, Rome, Italy
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  • Lorenzo Camoni

    Corresponding author
    1. Department of Biology, University of Rome “Tor Vergata”, Rome, Italy
    • Address correspondence to: Patrizia Aducci, Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy. or Lorenzo Camoni, Department of Biology, University of Rome “Tor Vergata”, via della Ricerca Scientifica, 00133 Rome, Italy; Tel., +39 06 72594343; Fax, +39 06 2023500.E-mail: aducci@uniroma2.it or E-mail: camoni@uniroma2.it.

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Abstract

Modulation of the interaction of regulatory 14-3-3 proteins to their physiological partners through small cell-permeant molecules is a promising strategy to control cellular processes where 14-3-3s are engaged. Here, we show that the fungal phytotoxin fusicoccin (FC), known to stabilize 14-3-3 association to the plant plasma membrane H+-ATPase, is able to stabilize 14-3-3 interaction to several client proteins with a mode III binding motif. Isothermal titration calorimetry analysis of the interaction between 14-3-3s and different peptides reproducing a mode III binding site demonstrated the FC ability to stimulate 14-3-3 the association. Moreover, molecular docking studies provided the structural rationale for the differential FC effect, which exclusively depends on the biochemical properties of the residue in peptide C-terminal position. Our study proposes FC as a promising tool to control cellular processes regulated by 14-3-3 proteins, opening new perspectives on its potential pharmacological applications. © 2014 IUBMB Life, 66(1):52–62, 2014

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