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High expression stability of microtubule affinity regulating kinase 3 (MARK3) makes it a reliable reference gene
Article first published online: 19 JAN 2010
DOI: 10.1002/iub.295
Copyright © 2010 International Union of Biochemistry and Molecular Biology, Inc.
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How to Cite
Chia, C.-Y., Lim, C. W., Leong, W.-T. and Ling, M. H. (2010), High expression stability of microtubule affinity regulating kinase 3 (MARK3) makes it a reliable reference gene. IUBMB Life, 62: 200–203. doi: 10.1002/iub.295
Publication History
- Issue published online: 12 MAR 2010
- Article first published online: 19 JAN 2010
- Manuscript Accepted: 30 NOV 2009
- Manuscript Received: 6 OCT 2009
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Keywords:
- reference gene;
- eukaryotic gene expression;
- mouse liver;
- microarray;
- MARK3
Abstract
Difference in gene expressions is characteristic of the function of different cell types and those genes with low expression variance can be used as standards for quantitative gene expression studies. Microarray technology is used to study global gene expression within a cell; hence, represents a suitable source of data to mine for genes with low expression variance. The coefficient of variation (COV) of each gene was determined and a threshold of less than 0.1 COV was used to select stably expressed genes in each data set. Our results showed that microtubule affinity-regulating kinase 3 (MARK3) has the lowest COV in eight microarray datasets. In addition, the gene expression of housekeeping genes, which is very likely to be stably expressed, tends to fluctuate highly under different conditions, marking them as being less reliable for use as reference genes. © 2010 IUBMB IUBMB Life, 62(3): 200–203, 2010