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ejp181-sup-0001-si.tif9677KFigure S1. Graphs depicting the effect of chronic constriction injury (CCI) of the left sciatic nerve on the mechanical and thermal threshold of the ipsilateral paw (A and C, respectively) and contralateral paw (B and D, respectively). In the ipsilateral paw, both mechanical (A) and thermal (B) hyperalgesia was observed at 7 days after the CCI surgery. No significant changes were observed in the contralateral paw. **p < 0.01 and ***p < 0.001 by two-way ANOVA followed by Bonferroni post hoc test.
ejp181-sup-0002-si.tif49084KFigure S2. Photomicrographs showing the co-localization of pERK1/2 (green) with NeuN, TH or TrpOH (red). Double labelling was used to evaluate the nature of the cells expressing pERK1/2 in spinal cord (neurons, co-localization with NeuN) and also to facilitate the delimitation of noradrenergic (A5, LC and A7, co-localization with TH) and serotonergic (ROb, RMg and DRN, co-localization with TrpOH) populations. Double-labelled cells are indicated by yellow colour in the merged figure (arrows). Scale bar = 100 μm in all fluorescent photomicrographs. Note the localization of DAB brownish precipitate in the perikarya, dendrites and nucleus of the neuron (right corner of figure). Scale bar = 10 μm.
ejp181-sup-0003-si.pdf12KTable S1. Table depicting the number of pERK1/2 IR cells per section for each spinal cord region analysed in the ipsilateral side in each experimental group. Values expressed as mean ± SEM p-values were obtained by two-way ANOVA followed by Fisher LSD post hoc test.
ejp181-sup-0004-si.pdf7KTable S2. Table depicting the number of pERK1/2 IR cells or the densitometric values (LC and LPB) per section for each brainstem nucleus studied in each experimental group. Values expressed as mean ± SEM p-values were obtained by two-way ANOVA followed by Fisher LSD post hoc test.
ejp181-sup-0005-si.docx40KAppendix S1. Additional discussion.

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