This paper presents the initial radiocarbon ages from the Lower Murray Archaeological Project developed in conjunction with the Ngarrindjeri Heritage Committee in South Australia. As part of this community based research program, a total of 59 shell (V. ambiguus) and charcoal samples were selected for radiocarbon dating. Radiocarbon ages varied significantly between sites within the region and ranged from 150–490 calBP at Pomberuk (Hume Reserve Midden and Historic Campsite) to 8190–8390 calBP at Glen Lossie Midden and Burial Site (GLMBS). These results will contribute to developing a more comprehensive understanding of human occupation in Ngarrindjeri ruwe (lands and waters) and assist in developing a local chronology for the region.