Diurnal Rhythm of Testosterone and Luteinizing Hormone in Hypogonadal Men

Authors


Montefiore Hospital, Department of Medicine, 3459 Fifth Avenue, Pittsburgh, Pennsylvania 15213.

Abstract

ABSTRACT: To determine the relationship between increasing luteinizing hormone (LH) production and the diurnal secretion of LH and testosterone (T) in adult men, studies were performed on five men with gonadotropin insufficiency associated with prolactinoma, five eugonadal men, and five men with primary testicular failure. Blood samples were drawn every 10 to 20 minutes for 24 hours beginning at 8:00 to 8:30 AM to evaluate diurnal periodicity. Mean (± SEM) LH levels in the three groups were 7.67 ± 1.46 mlU/ml, 13.9 ± 3.2 mlU/ml, and 62.3 ± 14.4 mlU/ml, respectively, and mean serum T levels were 8.05 ± 1.49 nmol/L, 13.9 ± 3.5 nmol/L, and 9.15 ± 1.3 nmol/L, respectively. Cosinor analysis revealed that each hyperprolactinemic man had a T rhythm with a significant 24-hour periodicity; the mean acrophase was at 5:00 AM. Testosterone levels were 35.0 ± 10.6% less at 4:00 PM than at 8:00 AM. Eugonadal men also demonstrated a significant diurnal T rhythm with an acrophase at 6:00 AM, and T levels were 15.8 ± 5.3% less at 4:00 PM than at 8:00 AM. By contrast, there was no significant diurnal rhythm in T secretion among the men with testicular failure, although serum T levels were 11.5 ± 3.7% less at 4:00 PM than at 8:00 AM. For LH, hyperprolactinemic men demonstrated a significant 24-hour rhythm with an acrophase at 1:30 AM, whereas no significant 24-hour periodicity was identified among either eugonadal men or men with testicular failure. The diurnal LH rhythm characteristic of pubertal boys is a property of the gonadotropin-receptor hormone (GnRH) pulse generator that is expressed in adult men with hyperprolactinemia and gonadotropin insufficiency. These data are consistent with the possibility that the expression of this rhythm reflects an enhanced gonadotropin-suppressive action of T in these men. As gonadotropin secretion increases to and beyond normal levels, there is a progressive dampening of the diurnal patterns of T and LH secretion.

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