Role of nuclear factor-kappa B activation in the adverse effects induced by air pollution particulate matter (PM2.5) in human epithelial lung cells (L132) in culture

Authors

  • Zeina Dagher,

    1. LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France
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  • Guillaume Garçon,

    1. LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France
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  • Sylvain Billet,

    1. LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France
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  • Anthony Verdin,

    1. LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France
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  • Frédéric Ledoux,

    1. LCE EA2598, Catalyse et Environnement, Maison de la Recherche en Environnement Industriel de Dunkerque 1, 145, Avenue Maurice Schumann, 59140 Dunkerque, France
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  • Dominique Courcot,

    1. LCE EA2598, Catalyse et Environnement, Maison de la Recherche en Environnement Industriel de Dunkerque 1, 145, Avenue Maurice Schumann, 59140 Dunkerque, France
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  • Antoine Aboukais,

    1. LCE EA2598, Catalyse et Environnement, Maison de la Recherche en Environnement Industriel de Dunkerque 1, 145, Avenue Maurice Schumann, 59140 Dunkerque, France
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  • Pirouz Shirali

    Corresponding author
    1. LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France
    • LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France.
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Abstract

ABSTRACT: To contribute to improving knowledge on the adverse health effects induced by particulate matter (PM) air pollution, an extensive investigation was undertaken of the underlying mechanisms of action activated by PM2.5 air pollution collected in Dunkerque, a strongly industrialized French seaside city. Their chemical and physical characteristics have been previously determined, and earlier in vitro short-term studies have shown them to cause dose-dependent and time-dependent oxidative damage, gene expression and protein secretion of inflammatory mediators, and apoptotic events in human lung epithelial cells (L132) in culture. Hence, this work studied the activation of nuclear factor-kappa B (NF-κB)/inhibitory kappa B (IκB) by Dunkerque city PM2.5 in these target cells, by determination of phosphorylated p65 and phosphorylated IκBα protein levels in cytoplasmic extracts, and p65 and p50 DNA binding in nuclear extracts. In PM-exposed L132 cells, there were concentration- and/or time-dependent increases in nuclear p65 and cytoplasmic IkB-α phosphorylation, and nuclear p65 and p50 DNA binding. Taken together, these results showed that Dunkerque city PM2.5 were involved in the activation of the NF-κB/IκB complex, notably through the occurrence of oxidative stress conditions, and, therefore, in the gene expression and protein secretion of inflammatory mediators in target L132 cells. Hence, these findings suggested that the activation of the NF-κB/IκB complex preceded cytotoxicity in Dunkerque city PM-exposed L132 cells. Copyright © 2007 John Wiley & Sons, Ltd.

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