Short Communication

Role of nuclear factor-kappa B activation in the adverse effects induced by air pollution particulate matter (PM2.5) in human epithelial lung cells (L132) in culture

  1. Zeina Dagher1,
  2. Guillaume Garçon1,
  3. Sylvain Billet1,
  4. Anthony Verdin1,
  5. Frédéric Ledoux2,
  6. Dominique Courcot2,
  7. Antoine Aboukais2,
  8. Pirouz Shirali1,*

Article first published online: 30 JAN 2007

DOI: 10.1002/jat.1211

Issue

Journal of Applied Toxicology

Journal of Applied Toxicology

Volume 27, Issue 3, pages 284–290, May/June 2007

Additional Information

How to Cite

Dagher, Z., Garçon, G., Billet, S., Verdin, A., Ledoux, F., Courcot, D., Aboukais, A. and Shirali, P. (2007), Role of nuclear factor-kappa B activation in the adverse effects induced by air pollution particulate matter (PM2.5) in human epithelial lung cells (L132) in culture. Journal of Applied Toxicology, 27: 284–290. doi: 10.1002/jat.1211

Author Information

  1. 1

    LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France

  2. 2

    LCE EA2598, Catalyse et Environnement, Maison de la Recherche en Environnement Industriel de Dunkerque 1, 145, Avenue Maurice Schumann, 59140 Dunkerque, France

*LCE EA2598, Toxicologie Industrielle et Environnementale, Maison de la Recherche en Environnement Industriel de Dunkerque 2, 189A, Avenue Maurice Schumann, 59140 Dunkerque, France.

Publication History

  1. Issue published online: 3 APR 2007
  2. Article first published online: 30 JAN 2007
  3. Manuscript Revised: 10 NOV 2006
  4. Manuscript Accepted: 10 NOV 2006
  5. Manuscript Received: 26 SEP 2006

Funded by

  • University of Littoral-Opale Coast
  • Franco-British Interreg IIIA European Program. Grant Number: French Convention no 24
  • Research Institute of Industrial Environment
  • French Ministry for Higher Education and Research. Grant Number: Convention no 16848-2005
  • Regional Council of Nord/Pas-de-Calais. Grant Number: Convention no 04 06 0092

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Keywords:

  • human lung epithelial cells (L132);
  • particulate matter;
  • physical and chemical characteristics;
  • oxidative stress;
  • NF-κB;
  • inflammation

Abstract

ABSTRACT: To contribute to improving knowledge on the adverse health effects induced by particulate matter (PM) air pollution, an extensive investigation was undertaken of the underlying mechanisms of action activated by PM2.5 air pollution collected in Dunkerque, a strongly industrialized French seaside city. Their chemical and physical characteristics have been previously determined, and earlier in vitro short-term studies have shown them to cause dose-dependent and time-dependent oxidative damage, gene expression and protein secretion of inflammatory mediators, and apoptotic events in human lung epithelial cells (L132) in culture. Hence, this work studied the activation of nuclear factor-kappa B (NF-κB)/inhibitory kappa B (IκB) by Dunkerque city PM2.5 in these target cells, by determination of phosphorylated p65 and phosphorylated IκBα protein levels in cytoplasmic extracts, and p65 and p50 DNA binding in nuclear extracts. In PM-exposed L132 cells, there were concentration- and/or time-dependent increases in nuclear p65 and cytoplasmic IkB-α phosphorylation, and nuclear p65 and p50 DNA binding. Taken together, these results showed that Dunkerque city PM2.5 were involved in the activation of the NF-κB/IκB complex, notably through the occurrence of oxidative stress conditions, and, therefore, in the gene expression and protein secretion of inflammatory mediators in target L132 cells. Hence, these findings suggested that the activation of the NF-κB/IκB complex preceded cytotoxicity in Dunkerque city PM-exposed L132 cells. Copyright © 2007 John Wiley & Sons, Ltd.

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