Diphenyl diselenide protects against glycerol-induced renal damage in rats
Article first published online: 29 MAY 2009
Copyright © 2009 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 29, Issue 7, pages 612–618, October 2009
How to Cite
Brandão, R., Acker, C. I., Leite, M. R., Barbosa, N. B. V. and Nogueira, C. W. (2009), Diphenyl diselenide protects against glycerol-induced renal damage in rats. J. Appl. Toxicol., 29: 612–618. doi: 10.1002/jat.1449
- Issue published online: 16 OCT 2009
- Article first published online: 29 MAY 2009
- Manuscript Accepted: 22 APR 2009
- Manuscript Revised: 16 APR 2009
- Manuscript Received: 3 FEB 2009
- acute renal failure;
- oxidative stress;
- sulfhydryl enzyme
In this study we evaluated the effect of diphenyl diselenide (PhSe)2 on glycerol-induced acute renal failure in rats. Rats were pre-treated by gavage every day with (PhSe)2 (7.14 mg kg−1) for 7 days. On the eighth day, rats received an intramuscular injection of glycerol (8 mL kg−1). Twenty-four hours afterwards, rats were euthanized and the levels of urea and creatinine were measured in plasma. Catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), δ-aminolevulinate dehydratase (δ-ALA-D) and Na+, K+-ATPase activities and ascorbic acid levels were evaluated in renal homogenates. Histopathological evaluations were also performed. The results demonstrated that (PhSe)2 was able to protect against the increase in urea and creatinine levels and histological alterations in kidney induced by glycerol. (PhSe)2 protected against the inhibition in δ-ALA-D, CAT and GPx activities and the reduction in ascorbic acid levels induced by glycerol in kidneys of rats. In conclusion, the present results indicate that (PhSe)2 was effective in protecting against acute renal failure induced by glycerol. Copyright © 2009 John Wiley & Sons, Ltd.