Cytotoxicity and biological effects of functional nanomaterials delivered to various cell lines
Article first published online: 16 SEP 2009
Copyright © 2009 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 30, Issue 1, pages 74–83, January 2010
How to Cite
Mahmood, M., Casciano, D. A., Mocan, T., Iancu, C., Xu, Y., Mocan, L., Iancu, D. T., Dervishi, E., Li, Z., Abdalmuhsen, M., Biris, A. R., Ali, N., Howard, P. and Biris, A. S. (2010), Cytotoxicity and biological effects of functional nanomaterials delivered to various cell lines. J. Appl. Toxicol., 30: 74–83. doi: 10.1002/jat.1475
- Issue published online: 16 DEC 2009
- Article first published online: 16 SEP 2009
- Manuscript Accepted: 27 JUL 2009
- Manuscript Revised: 5 JUL 2009
- Manuscript Received: 26 FEB 2009
- cancer cells;
- chemotherapeutic agents
Functional nanomaterials that included gold, silver nanoparticles and single wall carbon nanotubes were delivered to two cell lines (MLO-Y4 osteocytic cells and HeLa cervical cancer cells) in various concentrations. The cells were found to uptake the nanomaterials in a relatively short time, a process that significantly affected the shape and the size of the cells. The percentage of cellular death, due to the delivery of these nanomaterials, was found to be the highest for carbon nanotubes and increased gradually with the concentration of these nanostructures. Moreover, when the nanomaterials were delivered to the cells combined with commonly used chemotherapeutic agents such as etoposide or dexamethasone, the number of the cells that died increased significantly (100–300%) as compared with the case when only the nanomaterials or the chemotherapeutic agents were delivered. The experimental results were confirmed by Caspase 3 studies, indicating a strong interaction between the nanomaterials used in this study and the protein structure of the cells, which allowed a more effective action of the apoptotic agents. These findings could be the foundation of a new class of cancer therapies that are composed of both chemotherapeutic agents and nanomaterials. Copyright © 2009 John Wiley & Sons, Ltd.