Present address: Forensic Science Institute, Gujarat Forensic Sciences University, DFS Head Quarter, Sector 18/A, Near Police Bhavan, Gandhinagar-382007, Gujarat, India.
Detoxification and antioxidant effects of curcumin in rats experimentally exposed to mercury
Article first published online: 12 MAR 2010
Copyright © 2010 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 30, Issue 5, pages 457–468, July 2010
How to Cite
Agarwal, R., Goel, S. K. and Behari, J. R. (2010), Detoxification and antioxidant effects of curcumin in rats experimentally exposed to mercury. J. Appl. Toxicol., 30: 457–468. doi: 10.1002/jat.1517
- Issue published online: 19 JUL 2010
- Article first published online: 12 MAR 2010
- Manuscript Accepted: 1 FEB 2010
- Manuscript Revised: 29 JAN 2010
- Manuscript Received: 29 AUG 2009
- oxidative stress;
- antioxidant defense system;
- MT expression
Curcumin, a safe nutritional component and a highly promising natural antioxidant with a wide spectrum of biological functions, has been examined in several metal toxicity studies, but its role in protection against mercury toxicity has not been investigated. Therefore, the detoxification and antioxidant effects of curcumin were examined to determine its prophylactic/therapeutic role in rats experimentally exposed to mercury (in the from of mercuric chloride-HgCl2, 12 µmol kg−1 b.w. single intraperitoneal injection). Curcumin treatment (80 mg kg−1 b.w. daily for 3 days, orally) was found to have a protective effect on mercury-induced oxidative stress parameters, namely, lipid peroxidation and glutathione levels and superoxide dismutase, glutathione peroxidase and catalase activities in the liver, kidney and brain. Curcumin treatment was also effective for reversing mercury-induced serum biochemical changes, which are the markers of liver and kidney injury. Mercury concentration in the tissues was also decreased by the pre/post-treatment with curcumin. However, histopathological alterations in the liver and kidney were not reversed by curcumin treatment. Mercury exposure resulted in the induction of metallothionein (MT) mRNA expressions in the liver and kidney. Metallothionein mRNA expression levels were found to decrease after the pre-treatment with curcumin, whereas post-treatment with curcumin further increased MT mRNA expression levels. Our findings suggest that curcumin pretreatment has a protective effect and that curcumin can be used as a therapeutic agent in mercury intoxication. The study indicates that curcumin, an effective antioxidant, may have a protective effect through its routine dietary intake against mercury exposure.