Studies on the toxic effects of microcystin-LR on the zebrafish (Danio rerio) under different temperatures

Authors

  • Xuezhen Zhang,

    1. Fisheries College of Huazhong Agricultural University; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, People's Republic of China
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  • Wei Ji,

    1. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China
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  • Huan Zhang,

    1. Fisheries College of Huazhong Agricultural University; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, People's Republic of China
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  • Wei Zhang,

    1. Fisheries College of Huazhong Agricultural University; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, People's Republic of China
    2. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China
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  • Ping Xie

    Corresponding author
    1. Fisheries College of Huazhong Agricultural University; Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Wuhan 430070, People's Republic of China
    2. Donghu Experimental Station of Lake Ecosystems, State Key Laboratory of Freshwater Ecology and Biotechnology of China, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, People's Republic of China
    • Institute of Hydrobiology, Chinese Academy of Sciences, Donghu South Road 7, Wuhan 430072, People's Republic of China
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Abstract

It is well known that fish have stronger tolerance than mammals to microcystin (MC) exposure, and such a difference is attributed to their different core body temperatures. However, no in vivo study has been conducted to investigate the effects of temperature on MC-induced toxicity in fish, a typical poikilotherm. Tolerance and detoxification response of zebrafish treated with MC-LR were investigated under three temperatures. The LD50 values evidently increased with a decline of the temperature (547, 260 and 176 µg kg−1 at 12, 22 and 32 °C, respectively), indicating stronger tolerance of the fish at lower temperatures. Changes in the transcription of glutathione S-transferase (GST) isoforms in the fish were observed, and their sensitivity of response in the transcription of GST mRNA was on the order of 12 > 32 > 22°C. We screened out several GST genes which were more delicate to solve the MC-LR exposure at different temperatures, i.e. GST rho1, al, p1 and theta1 in the 12 °C group, and GST zeta1 and p2 in the 22 and 32 °C groups. Our findings partly validate the hypothesis that high temperature enhances toxic effects of MCs on poikilotherms. Our studies also indicate that temperature-dependent toxic effects should be taken into account for field toxic assessment of microcystins in fish. Copyright © 2010 John Wiley & Sons, Ltd.

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