These authors contributed equally to this manuscript.
In vitro antioxidant and antigenotoxic potentials of 3,5-O-di-galloylquinic acid extracted from Myrtus communis leaves and modulation of cell gene expression by H2O2
Article first published online: 13 JUL 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 32, Issue 5, pages 333–341, May 2012
How to Cite
Ines, S., Ines, B., Wissem, B., Mohamed, B. S., Nawel, H., Dijoux-Franca, M.-G., Kamel, G. and Leïla, C.-G. (2012), In vitro antioxidant and antigenotoxic potentials of 3,5-O-di-galloylquinic acid extracted from Myrtus communis leaves and modulation of cell gene expression by H2O2. J. Appl. Toxicol., 32: 333–341. doi: 10.1002/jat.1655
- Issue published online: 23 MAR 2012
- Article first published online: 13 JUL 2011
- Manuscript Accepted: 14 DEC 2010
- Manuscript Revised: 12 NOV 2010
- Manuscript Received: 10 JUL 2010
- 3,5-O-di-galloylquinic acid;
3,5-O-di-galloylquinic acid (DGQA) purified from leaves of Myrtus communis was investigated for its antioxidative, antiproliferative and antigenotoxic activities. Antioxidant activity was determined by the ability of the compound to inhibit lipid peroxidation induced by H2O2 in the K562 cell line. The pure molecule displayed an important malondialdehyde formation inhibition percentage (82.2%). Moreover, this compound exhibited an inhibitory effect against H2O2-induced genotoxicity, using the comet assay. A protective effect of the same molecule was also revealed when assessing the gene expression of the chronic myelogenous leukemia cell line (K562), stressed with H2O2. For this purpose, we used a cDNA-microarray containing 82 genes related to cell defense, essentially represented by antioxidant and DNA repair proteins. We found that DGQA increase the activity of antioxidant enzymes family and the activity of DNA repair enzymes. Taken together, these observations provide evidence that the DGQA is able to protect cells against oxidative stress.