Nandrolone androgenic hormone presents genotoxic effects in different cells of mice

Authors

  • Carolina Almeida do Carmo,

    1. Programa de Pós-Graduação em Biologia Geral e Aplicada, Universidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatu, São Paulo, Brazil
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  • Álvaro Luiz Martini Gonçalves,

    1. Programa de Pós-Graduação em Biologia Geral e Aplicada, Universidade Estadual Paulista (UNESP), Instituto de Biociências, Botucatu, São Paulo, Brazil
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  • Daisy Maria Fávero Salvadori,

    1. Departamento de Patologia, Faculdade de Medicina, Universidade Estadual Paulista (UNESP), Botucatu, São Paulo, Brazil
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  • Edson Luis Maistro

    Corresponding author
    • Universidade Estadual Paulista – UNESP – Faculdade de Filosofia e Ciências, Departamento de Fonoaudiologia, Marília, SP, Brazil
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E. L. Maistro, Universidade Estadual Paulista – UNESP – Faculdade de Filosofia e Ciências, Departamento de Fonoaudiologia, Marília, SP 17525-900, Brazil.

E-mail: edson.maistro@marilia.unesp.br

ABSTRACT

Nandrolone is an androgenic–anabolic steroid (AAS) with diverse medical applications but taken indiscriminately by some to rapidly increase muscle mass. The aim of this study was to evaluate the genotoxic and clastogenic potential of nandrolone (deca-durabolin®) in vivo in different cells of mice, using the comet assay and micronucleus test, respectively. The animals received subcutaneous injection of the three doses of the steroid (1.0, 2.5 and 5.0 mg kg−1 body weight). Cytotoxicity was assessed by scoring 200 consecutive total polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE–NCE ratio). The results showed a significant dose-related increase in the frequency of DNA damage in leukocytes, liver, bone marrow, brain and testicle cells at the three tested doses and a significant increase of the micronucleated polychromatic erythrocytes at all tested doses. Under our experimental conditions, the nandrolone steroid hormone showed genotoxic and clastogenic effects when administered subcutaneously to mice. Copyright © 2011 John Wiley & Sons, Ltd.

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