The research was presented in part at the 44th Congress of the European Societies of Toxicology, Amsterdam, 7–10 October 2007.
Hyaline droplet accumulation in kidney of rats treated with hexachloro-1:3-butadiene: influence of age, dose and time-course†
Article first published online: 12 SEP 2011
Copyright © 2011 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 33, Issue 3, pages 183–189, March 2013
How to Cite
Cristofori, P., Defazio, R., Chiusolo, A., Mongillo, M., Bartolucci, G. B., Chiara, F. and Trevisan, A. (2013), Hyaline droplet accumulation in kidney of rats treated with hexachloro-1:3-butadiene: influence of age, dose and time-course. J. Appl. Toxicol., 33: 183–189. doi: 10.1002/jat.1732
- Issue published online: 25 JAN 2013
- Article first published online: 12 SEP 2011
- Manuscript Revised: 3 AUG 2011
- Manuscript Accepted: 3 AUG 2011
- Manuscript Received: 15 JUL 2011
- hyaline droplet;
The present research investigates the occurrence of hyaline droplet (HD) accumulation related to age, dose and time after treatment in male Wistar rats given a single i.p. injection of hexachloro-1:3-butadiene (HCBD). In the study on age, rats from 1 to 12 months of age were treated with 100 mg kg−1 body weight (b.w.) HCBD dose. Rats treated at 2 months of age showed a greater accumulation of HD than the other age groups; HD accumulation was not observed in 1-month-old rats. In the dose–response study, the treatment with 25, 50 and 100 mg kg−1 b.w. at 2 months of age caused HD accumulation in the proximal convoluted tubule at all doses, with the 100 mg kg−1 b.w. group slightly more affected. Finally, in the time-course study, rats treated with a 100 mg kg−1 b.w. dose at 2 months of age and sacrificed at 6, 12, 24, 48, 72 and 96 h post-dosing showed a time-related HD accumulation in terms of incidence and severity, after 6 h, with a peak at 24 and 48 h and decreasing at 72 and 96 h. The present results show that HD accumulation is an early finding, and is unrelated to dose level and particularly evident in rats of 2 month of age. These findings in male rats treated with HCBD emphasize the importance of considering the age of rats at the start of a study. The more sensitive model was used in the detection of nephrotoxic effects of chemicals. Copyright © 2011 John Wiley & Sons, Ltd.