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Homologous recombination induced by doxazosin mesylate and saw palmetto in the Drosophila wing-spot test

Authors

  • Katiane Cella Gabriel,

    1. Laboratório da Toxicidade Genética, Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil, Canoas, RS, Brazil
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  • Rafael Rodrigues Dihl,

    Corresponding author
    • Laboratório da Toxicidade Genética, Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil, Canoas, RS, Brazil
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  • Mauricio Lehmann,

    1. Laboratório da Toxicidade Genética, Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil, Canoas, RS, Brazil
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  • Maria Luiza Reguly,

    1. Laboratório da Toxicidade Genética, Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil, Canoas, RS, Brazil
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  • Marc François Richter,

    1. Laboratório de Fitoquímica, Programa de Pós-Graduação em Genética e Toxicologia Aplicada, Universidade Luterana do Brasil, Canoas, RS, Brazil
    2. Curso de Biologia Marinha e Costeira, Universidade Estadual do Rio Grande do Sul, Porto Alegre, Brazil
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  • Heloisa Helena Rodrigues de Andrade

    1. Laboratório de Estomatologia, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
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Correspondence to: Rafael Rodrigues Dihl, Laboratório da Toxicidade Genética, ULBRA, Prédio 22, 4o andar, Avenida Farroupilha, 8001, 92420–280, Canoas, RS, Brazil. E-mail: rafael.rodrigues@ulbra.br

ABSTRACT

Benign prostatic hyperplasia (BPH) is the most common tumor in men over 40 years of age. Acute urinary retention (AUR) is regarded as the most serious hazard of untreated BPH. α-Blockers, such as doxazosin mesylate, and 5-α reductase inhibitors, such as finasteride, are frequently used because they decrease both AUR and the need for BPH-related surgery. An extract of the fruit from American saw palmetto plant has also been used as an alternative ttreatment for BPH. The paucity of information available concerning the genotoxic action of these compounds led us to assess their activity as inducers of different types of DNA lesions using the somatic mutation and recombination test in Drosophila melanogaster. Finasteride did not induce gene mutation, chromosomal mutation or mitotic recombination, which means it was nongenotoxic in our experimental conditions. On the other hand, doxazosin mesylate and saw palmetto induced significant increases in spot frequencies in trans-heterozygous flies. In order to establish the actual role played by mitotic recombination and by mutation in the genotoxicity observed, the balancer-heterozygous flies were also analyzed, showing no increment in the total spot frequencies in relation to the negative control, for both drugs. Doxazosin mesylate and saw palmetto were classified as specific inducers of homologous recombination in Drosophila proliferative cells, an event linked to the loss of heterozygosity. Copyright © 2011 John Wiley & Sons, Ltd.

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