In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage
Article first published online: 4 APR 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 33, Issue 9, pages 880–885, September 2013
How to Cite
Casanova, N. A., Ariagno, J. I., López Nigro, M. M., Mendeluk, G. R., Gette, M. d. l. A., Petenatti, E., Palaoro, L. A. and Carballo, M. A. (2013), In vivo antigenotoxic activity of watercress juice (Nasturtium officinale) against induced DNA damage. J. Appl. Toxicol., 33: 880–885. doi: 10.1002/jat.2746
- Issue published online: 23 JUL 2013
- Article first published online: 4 APR 2012
- Manuscript Accepted: 5 FEB 2012
- Manuscript Revised: 3 FEB 2012
- Manuscript Received: 24 NOV 2011
The present study was carried out to investigate the genotoxicity as well as possible protective activity against damage induced by cyclophosphamide (CP) of the aqueous juice of watercress (Nasturtium officinale, W.T. Aiton) in vivo. Male and female Swiss mice 7–8 weeks old (N = 48) were treated by gavage with 1 g kg−1 body weight and 0.5 g kg−1 body weight of watercress juice during 15 consecutive days. Genotoxicity and its possible protective effect were tested by the comet assay in peripheral blood cells and the micronucleus test in bone marrow. In addition, biopsies of the bladder, epididymis and testicles of mice were performed to extend the experimental design. Watercress juice per se did not induce genetic damage according to the comet assay and micronucleus study, exhibiting a protective activity against CP (P < 0.05 and P < 0.001, respectively). The comparative analysis of bladder histological changes obtained in the watercress plus CP group against those treated with CP alone suggests a probable protective effect. Further studies are needed in order to establish the protective role of watercress juice against DNA damage. Copyright © 2012 John Wiley & Sons, Ltd.