Nanoparticulate silver increases uric acid and allantoin excretion in rats, as identified by metabolomics
Article first published online: 18 MAY 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 32, Issue 11, pages 929–933, November 2012
How to Cite
Hadrup, N., Lam, H. R., Loeschner, K., Mortensen, A., Larsen, E. H. and Frandsen, H. (2012), Nanoparticulate silver increases uric acid and allantoin excretion in rats, as identified by metabolomics. J. Appl. Toxicol., 32: 929–933. doi: 10.1002/jat.2779
- Issue published online: 26 SEP 2012
- Article first published online: 18 MAY 2012
- Manuscript Revised: 18 APR 2012
- Manuscript Accepted: 18 APR 2012
- Manuscript Received: 2 FEB 2012
- uric acid
Metabolomic investigation of rat urine was employed to identify mammalian metabolites affected by ionic or nanoparticulate silver. Female and male Wistar rats were administered silver nanoparticles (2.25, 4.5 or 9.0 mg kg−1 body weight per day) or ionic silver (silver acetate, 9.0 mg silver kg−1 bw per day) by oral gavage for 28 days. On day 18, urine was collected for 24 h and subjected to metabolomics with high performance liquid chromatography–quadropole time-of-flight mass spectrometry (HPLC-QTOF-MS)-based separation and detection. Principal component analysis was subsequently applied to the data. Metabolomic differences in urine composition were found in female rats but not in male rats. Several metabolites were identified by the use of elemental composition calculated from the exact mass combined with searches in the Human Metabolome Database.The metabolite identities were eventually verified by co-chromatography with authentic standards. Differences were found in uric acid and its degradation product, allantoin. Administration of nanoparticulate silver increased both metabolites, whereas ionic silver only increased allantoin. In conclusion, metabolomic investigation of rat urine showed that increased levels of uric acid and allantoin were associated with exposure to nanoparticulate silver. Copyright © 2012 John Wiley & Sons, Ltd.