These authors contributed equally to this work.
Metabolic profiling reveals disorder of carbohydrate metabolism in mouse fibroblast cells induced by titanium dioxide nanoparticles
Article first published online: 20 SEP 2012
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 33, Issue 12, pages 1442–1450, December 2013
How to Cite
Jin, C., Liu, Y., Sun, L., Chen, T., Zhang, Y., Zhao, A., Wang, X., Cristau, M., Wang, K. and Jia, W. (2013), Metabolic profiling reveals disorder of carbohydrate metabolism in mouse fibroblast cells induced by titanium dioxide nanoparticles. J. Appl. Toxicol., 33: 1442–1450. doi: 10.1002/jat.2808
- Issue published online: 22 OCT 2013
- Article first published online: 20 SEP 2012
- Manuscript Revised: 2 JUL 2012
- Manuscript Accepted: 2 JUL 2012
- Manuscript Received: 10 MAY 2012
- TiO2 nanoparticles;
- metabolomic strategy;
- carbohydrate metabolism;
- L929 cells
As titanium dioxide (TiO2) nanoparticles are widely used commercially, their potential biosafety and metabolic mechanism needs to be fully explained. In this study, the cytotoxicity of homogeneous and weakly aggregated (< 100 nm) TiO2 nanoparticles was investigated by analyzing the changes in metabolite profiles both in mouse fibroblast (L929) cells and their corresponding culture media using gas chromatograph with a time-of-flight mass spectrometry (GC/TOFMS)-based metabolomic strategy. With multivariate statistics analysis, satisfactory separations were observed in principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) models. Based on the variable importance in the OPLS-DA models, a series of differential metabolites were identified by comparison between TiO2 nanoparticle-treated L929 cells or their corresponding culture media and the control groups. It was found that the major biochemical metabolism (carbohydrate metabolism) was suppressed in TiO2 nanoparticle-treated L929 cells and their corresponding culture media. These results might account for the serious damage to energy metabolism in mitochondria and the increased cellular oxidation stress in TiO2 nanoparticle-induced L929 cells. These results also suggest that the metabolomic strategy had a great potential in evaluating the cytotoxicity of TiO2 nanoparticles and thus was very helpful in understanding its underlying molecular mechanisms. Copyright © 2012 John Wiley & Sons, Ltd.