These two authors have contributed equally to the manuscript.
Acute toxicity of zinc oxide nanoparticles to the rat olfactory system after intranasal instillation
Article first published online: 11 JAN 2013
Copyright © 2012 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 33, Issue 10, pages 1079–1088, October 2013
How to Cite
Gao, L., Yang, S.-T., Li, S., Meng, Y., Wang, H. and Lei, H. (2013), Acute toxicity of zinc oxide nanoparticles to the rat olfactory system after intranasal instillation. J. Appl. Toxicol., 33: 1079–1088. doi: 10.1002/jat.2842
- Issue published online: 26 JUL 2013
- Article first published online: 11 JAN 2013
- Manuscript Revised: 24 OCT 2012
- Manuscript Accepted: 24 OCT 2012
- Manuscript Received: 11 MAR 2012
- China Ministry of Science and Technology. Grant Numbers: 2011CB933402, 2006CB705600
- Natural Science Foundation of China. Grant Numbers: 20921004, 2082010235
- zinc oxide;
- olfactory epithelium;
- magnetic resonance imaging
With the increased applications of zinc oxide (ZnO) nanoparticles (NPs), the toxicity of ZnO NPs arouses great concerns from the nano community and the general public. In this study, we report the toxicity of ZnO NPs (30 nm) to the rat olfactory system after intranasal instillation revealed by non-invasive magnetic resonance imaging (MRI). MRI scans were performed on a 4.7-T scanner at 1, 2, 3 and 7 days post-exposure, and the histological changes of the rat olfactory epithelium were evaluated. The influences of chemical component and dispersant of the NPs were also investigated. In addition, an olfactory behavior test was performed. The MRI and histological results indicated that ZnO NPs induced significant damages to the olfactory epithelium, including disruption of the olfactory epithelial structures and inflammation. The destruction of mitochondria in epithelial cells was observed under transmission electron microscopy (TEM), suggesting that the possible toxicological mechanism might involve cellular energy metabolic dysfunction. Further, the lesion of the olfactory epithelium disturbed sniffing behaviors of the treated animals. The results suggest that MRI is potentially useful as a screening tool to assess the consequence of occupational exposure of ZnO NPs. Caution should therefore be taken during the use and disposal of ZnO NPs to prevent the unintended public health impacts. Copyright © 2012 John Wiley & Sons, Ltd.