Nanosilver suppresses growth and induces oxidative damage to DNA in Caenorhabditis elegans

Authors

  • Piper Reid Hunt,

    Corresponding author
    • United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Division of Toxicology, Laurel, MD, USA
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  • Bryce J. Marquis,

    1. National Institute of Standards and Technology, Material Measurement Laboratory, Gaithersburg, MD, USA
    Current affiliation:
    1. University of Central Arkansas, Department of Chemistry, Conway, AR, USA
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  • Katherine M. Tyner,

    1. United States Food and Drug Administration, Center for Drug Evaluation & Research, Silver Spring, MD, USA
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  • Sean Conklin,

    1. United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Sciences, College Park, MD, USA
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  • Nicholas Olejnik,

    1. United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Division of Toxicology, Laurel, MD, USA
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  • Bryant C. Nelson,

    1. National Institute of Standards and Technology, Material Measurement Laboratory, Gaithersburg, MD, USA
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  • Robert L. Sprando

    1. United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Division of Toxicology, Laurel, MD, USA
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Correspondence to: Piper Reid Hunt, United States Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Division of Toxicology, Laurel, MD 20708, USA. E-mail: Piper.Hunt@fda.hhs.gov

ABSTRACT

Studies on the effects of nanomaterial exposure in mammals are limited, and new methods for rapid risk assessment of nanomaterials are urgently required. The utility of Caenorhabditis elegans cultured in axenic liquid media was evaluated as an alternative in vivo model for the purpose of screening nanomaterials for toxic effects. Spherical silver nanoparticles of 10 nm diameter (10nmAg) were used as a test material, and ionic silver from silver acetate as a positive control. Silver uptake and localization, larval growth, morphology and DNA damage were utilized as endpoints for toxicity evaluation. Confocal reflection analysis indicated that 10nmAg localized to the lumen and tissues of the digestive tract of C. elegans. 10nmAg at 10 µg ml–1 reduced the growth of C. elegans larvae, and induced oxidative damage to DNA as measured by 8-OH guanine levels. Consistent with previously published studies using mammalian models, ionic silver suppressed growth in C. elegans larvae to a greater extent than 10nmAg. Our data suggest that medium-throughput growth screening and DNA damage analysis along with morphology assessments in C. elegans could together provide powerful tools for rapid toxicity screening of nanomaterials. Published 2013. This article is a US Government work and is in the public domain in the USA.

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