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Molecular impact of juvenile hormone agonists on neonatal Daphnia magna

Authors

  • Kenji Toyota,

    1. Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, Japan
    2. Department of Basic Biology, Faculty of Life Science, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan
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  • Yasuhiko Kato,

    1. Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan
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  • Hitoshi Miyakawa,

    1. Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, Japan
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  • Ryohei Yatsu,

    1. Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, Japan
    2. Department of Basic Biology, Faculty of Life Science, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan
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  • Takeshi Mizutani,

    1. Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, Japan
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  • Yukiko Ogino,

    1. Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, Japan
    2. Department of Basic Biology, Faculty of Life Science, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan
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  • Shinichi Miyagawa,

    1. Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, Japan
    2. Department of Basic Biology, Faculty of Life Science, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan
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  • Hajime Watanabe,

    1. Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan
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  • Hiroyo Nishide,

    1. Data Integration and Analysis Facility, National Institute for Basic Biology, Okazaki, Aichi, Japan
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  • Ikuo Uchiyama,

    1. Data Integration and Analysis Facility, National Institute for Basic Biology, Okazaki, Aichi, Japan
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  • Norihisa Tatarazako,

    1. Environmental Quality Measurement Section, Research Center for Environmental Risk, National Institute for Environmental Studies, Tsukuba, Ibaraki, Japan
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  • Taisen Iguchi

    Corresponding author
    1. Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, Japan
    2. Department of Basic Biology, Faculty of Life Science, Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, Japan
    • Correspondence to: Taisen Iguchi, Okazaki Institute for Integrative Bioscience, National Institute for Basic Biology, National Institutes of Natural Sciences, 5–1 Higashiyama, Myodaiji, Okazaki, Aichi 444–8787, Japan.

      Email: taisen@nibb.ac.jp

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ABSTRACT

Daphnia magna has been used extensively to evaluate organism- and population-level responses to pollutants in acute toxicity and reproductive toxicity tests. We have previously reported that exposure to juvenile hormone (JH) agonists results in a reduction of reproductive function and production of male offspring in a cyclic parthenogenesis, D. magna. Recent advances in molecular techniques have provided tools to understand better the responses to pollutants in aquatic organisms, including D. magna. DNA microarray was used to evaluate gene expression profiles of neonatal daphnids exposed to JH agonists: methoprene (125, 250 and 500 ppb), fenoxycarb (0.5, 1 and 2 ppb) and epofenonane (50, 100 and 200 ppb). Exposure to these JH analogs resulted in chemical-specific patterns of gene expression. The heat map analyses based on hierarchical clustering revealed a similar pattern between treatments with a high dose of methoprene and with epofenonane. In contrast, treatment with low to middle doses of methoprene resulted in similar profiles to fenoxycarb treatments. Hemoglobin and JH epoxide hydrolase genes were clustered as JH-responsive genes. These data suggest that fenoxycarb has high activity as a JH agonist, methoprene shows high toxicity and epofenonane works through a different mechanism compared with other JH analogs, agreeing with data of previously reported toxicity tests. In conclusion, D. magna DNA microarray is useful for the classification of JH analogs and identification of JH-responsive genes. Copyright © 2013 John Wiley & Sons, Ltd.

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