Rapid determination of quetiapine in blood by gas chromatography–mass spectrometry. Application to post-mortem cases
Article first published online: 11 OCT 2013
Copyright © 2013 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
How to Cite
López-Guarnido, O., Tabernero, M. J., Hernández, A. F., Rodrigo, L. and Bermejo, A. M. (2013), Rapid determination of quetiapine in blood by gas chromatography–mass spectrometry. Application to post-mortem cases. J. Appl. Toxicol.. doi: 10.1002/jat.2944
- Article first published online: 11 OCT 2013
- Manuscript Revised: 9 SEP 2013
- Manuscript Accepted: 9 SEP 2013
- Manuscript Received: 26 JUN 2013
- blood analysis;
- liquid-liquid extraction;
- forensic toxicology
A simple, fast and sensitive method for the determination of quetiapine in human blood has been developed and validated. The method involved a basic liquid–liquid extraction procedure and subsequent analysis by gas chromatography–mass spectrometry, previous derivatization with bis(trimethylsilyl)-trifluoro-acetamide and chorotrimethylsilane (99 : 1). The methods of validation included linearity with a correlation coefficient > 0.99 over the range 0.02–1 µg ml–1, intra- and interday precision (always < 12%) and accuracy (mean relative error always < 12%) to meet the bioanalytical acceptance criteria. The limit of detection was 0.005 µg ml–1. The procedure was further applied to post mortems from the Institute of Legal Medicine, University of Santiago de Compostela. Copyright © 2013 John Wiley & Sons, Ltd.