Interaction of citrate-coated silver nanoparticles with earthworm coelomic fluid and related cytotoxicity in Eisenia andrei
Article first published online: 16 FEB 2014
Copyright © 2014 John Wiley & Sons, Ltd.
Journal of Applied Toxicology
Volume 34, Issue 11, pages 1145–1154, November 2014
How to Cite
2014) Interaction of citrate-coated silver nanoparticles with earthworm coelomic fluid and related cytotoxicity in Eisenia andrei, J. Appl. Toxicol., 34, 1145–1154, doi: 10.1002/jat.2993., , , and (
- Issue published online: 28 SEP 2014
- Article first published online: 16 FEB 2014
- Manuscript Revised: 24 DEC 2013
- Manuscript Accepted: 24 DEC 2013
- Manuscript Received: 2 JUL 2013
- silver nanoparticle;
- earthworm coelomic fluid;
- Eisenia andrei, biofluid
Understanding the interaction of nanoparticles with biological fluid is important for predicting the behavior and toxicity of nanoparticles in living systems. The earthworm Eisenia andrei was exposed to citrate-coated silver nanoparticles (cAgNPs), and the interaction of cAgNPs with earthworm coelomic fluid (ECF), the cytotoxicity of cAgNPs in earthworm coelomocytes was assessed. The neutral red retention assay showed a reduction in lysosomal stability after exposure. The toxicity of silver ions dissolved from cAgNPs in the soil medium was not significant. The aggregation and dissolution of cAgNPs increased in ECF, which contains various electrolytes that alter the properties of nanoparticles, and their subsequent toxicity. Microscopic and dissolution studies demonstrated that the aggregation of cAgNPs rapidly increased, and readily dissolved in ECF. The bioavailability of cAgNPs to earthworms induced lysosomal cytotoxicity. This is the first report to test the interaction and lysosomal cytotoxicity of nanoparticles in earthworm biofluids. Copyright © 2014 John Wiley & Sons, Ltd.