Inter-laboratory validation of the modified murine local lymph node assay based on 5-bromo-2′-deoxyuridine incorporation (pages 63–74)
Hajime Kojima, Masahiro Takeyoshi, Takashi Sozu, Takumi Awogi, Kazunori Arima, Kenji Idehara, Yoshiaki Ikarashi, Yukiko Kanazawa, Eiji Maki, Takashi Omori, Atsuko Yuasa and Isao Yoshimura
Article first published online: 30 JUL 2010 | DOI: 10.1002/jat.1567
Takeyoshi M. et al. (2001) has developed a modified murine local lymph node assay (LLNA) based on the 5-bromo-2'-deoxyuridine (BrdU) incorporation (LLNA:BrdU-ELISA). We conducted the validation study to evaluate the reliability and relevance of LLNA:BrdU-ELISA. The experiment involved 7 laboratories, wherein 10 chemicals were examined under blinded conditions. The validation results indicate that the LLNA:BrdU-ELISA is a promising new version LLNA.