Inhibitory effect of pisosterol on human glioblastoma cell lines with C-MYC amplification (pages 554–560)
Edmundo L. R. Pereira, Patrícia D. L. Lima, André S. Khayat, Marcelo O. Bahia, Franciglauber S. Bezerra, Manoel Andrade-Neto, Raquel C. Montenegro, Cláudia Pessoa, Letícia V. Costa-Lotufo, Manoel O. Moraes, France K. N. Yoshioka, Giovanny R. Pinto and Rommel R. Burbano
Article first published online: 9 NOV 2010 | DOI: 10.1002/jat.1596
We conducted a study in U343 and AHOL1 glioblastoma cell lines, before and after treatment with the triterpene pisosterol. Using fluorescence in situ hybridization analysis we showed that 72% of U343 and 65% of AHOL1 cells contained more than two alleles of C-MYC before treatment. However, at 1.8 µg ml−1 of pisosterol, only 33% of U343 cells and 15% of AHOL1 cells presented more than two fluorescent signals, suggesting that pisosterol blocks the cells with gene amplification.