Embryotoxicity assessment of developmental neurotoxicants using a neuronal endpoint in the embryonic stem cell test (pages 617–626)
Dae Hyun Baek, Tae Gyun Kim, Hwa Kyung Lim, Jin Wook Kang, Su Kyoung Seong, Seung Eun Choi, So Yun Lim, Sung Hee Park, Bong-hyun Nam, Eun Hee Kim, Mun Sin Kim and Kui Lea Park
Article first published online: 1 DEC 2011 | DOI: 10.1002/jat.1747
The embryonic stem cell test (EST) is a validated in vitro embryotoxicity test; however, as the inhibition of cardiac differentiation alone is used as a differentiation endpoint in the EST, it may not be a useful test to screen embryotoxic chemicals that affect the differentiation of noncardiac tissues. Previously, methylmercury (MeHg), cadmium and arsenic compounds, which are heavy metals that induce developmental neurotoxicity in vivo, were misclassified as nonembryotoxic with the EST.