Establishment and intra-/inter-laboratory validation of a standard protocol of reactive oxygen species assay for chemical photosafety evaluation (pages 1241–1250)
Satomi Onoue, Kazuhiro Hosoi, Shinobu Wakuri, Yumiko Iwase, Toshinobu Yamamoto, Naoko Matsuoka, Kazuichi Nakamura, Tsuguto Toda, Hironori Takagi, Naoto Osaki, Yasuhiro Matsumoto, Satoru Kawakami, Yoshiki Seto, Masashi Kato, Shizuo Yamada, Yasuo Ohno and Hajime Kojima
Article first published online: 13 JUN 2012 | DOI: 10.1002/jat.2776
In three participating laboratories, photochemical reactivities of 42 coded chemicals and two standard controls were assessed by reactive oxygen species (ROS) assay for photosafety evaluation. Outcomes from the validation study were indicative of satisfactory transferability, intra- and inter-laboratory variability, and predictive capacity of the ROS assay. Thus, results from the present validation study provided sufficient support for the ROS assay as an alternative method for photosafety assessment.