Establishment of transactivation assay systems using fish, amphibian, reptilian and human thyroid hormone receptors (pages 991–1000)
Tomohiro Oka, Naoko Mitsui-Watanabe, Norihisa Tatarazako, Yuta Onishi, Yoshinao Katsu, Shinichi Miyagawa, Yukiko Ogino, Ryohei Yatsu, Satomi Kohno, Minoru Takase, Yukio Kawashima, Yasuhiko Ohta, Yasunobu Aoki, Louis J. Guillette Jr and Taisen Iguchi
Article first published online: 30 OCT 2012 | DOI: 10.1002/jat.2825
Thyroid hormones are essential for the regulation of a wide range of biological processes associated with normal development and metabolism in vertebrates. For the screening of chemicals having potential thyroid hormone and anti-thyroid hormone activities, we have established transient transactivation assay systems. We demonstrate that this in vitro transactivation system can be used for screening chemicals with potential thyroid hormone agonistic and antagonistic activities.