Perturbation of cytosolic calcium by 2-aminoethoxydiphenyl borate and caffeine affects zebrafish myofibril alignment (pages 287–294)
Hsin-Ju Wu, Tsorng-Harn Fong, Shen-Liang Chen, Jen-Cheng Wei, I-Jong Wang, Chi-Chung Wen, Chao-Yuan Chang, Xing-Guang Chen, Wei-Yu Chen, Hui-Min Chen, Juin-Lin Horng, Yun-Hsin Wang and Yau-Hung Chen
Article first published online: 4 SEP 2014 | DOI: 10.1002/jat.3057
The objective of the current study was to investigate the correlation of cytosolic calcium levels and myofibril alignments during zebrafish embryogenesis. We exposed 2-aminoethoxydiphenyl borate and caffeine to zebrafish embryos to disturb the cytosolic calcium levels. Our data show that (1) both enhanced and reduced of cytosolic calcium led to mis-alignment of myofibrils; (2) reduction of cytosolic calcium increases myod expression; and (3) electron density is affected by intracellular calcium reduction. In conclusion, we proposed that cytosolic calcium is important for myofibrils alignment.