Are zebrafish larvae suitable for assessing the hepatotoxicity potential of drug candidates? (pages 1017–1029)
Natalie Mesens, Alexander D. Crawford, Aswin Menke, Pham Duc Hung, Freddy Van Goethem, Rik Nuyts, Erik Hansen, Andre Wolterbeek, Jacky Van Gompel, Peter De Witte and Camila V. Esguerra
Article first published online: 6 FEB 2015 | DOI: 10.1002/jat.3091
We evaluated lfabp10a as an endpoint for assessing the hepatotoxic effects in zebrafish larvae, and expression analysis was found to be a valid marker, as statistical significant abnormal lfabp10 expression levels correlated with hepatocellular histopathological changes. To assess the applicability for assessing human relevant DILI, 14 drugs were tested that have been marketed for human use, classified according to their mechanism of toxicity. The zebrafish larva showed promising predictivity and was capable of distinguishing between hepatotoxic and non-hepatotoxic chemical analogues.