Full Article

Effect of temperature on permeation of low-density lipoprotein particles through human carotid artery tissues

  1. Mohamad G. Ghosn1,
  2. Michael Leba1,
  3. Astha Vijayananda1,
  4. Panteha Rezaee1,
  5. Joel D. Morrisett2,
  6. Kirill V. Larin1,*

Article first published online: 12 MAR 2009

DOI: 10.1002/jbio.200810071

Issue

Journal of Biophotonics

Journal of Biophotonics

Volume 2, Issue 10, pages 573–580, October 2009

Additional Information

How to Cite

Ghosn, M. G., Leba, M., Vijayananda, A., Rezaee, P., Morrisett, J. D. and Larin, K. V. (2009), Effect of temperature on permeation of low-density lipoprotein particles through human carotid artery tissues. Journal of Biophotonics, 2: 573–580. doi: 10.1002/jbio.200810071

Author Information

  1. 1

    Biomedical Engineering Program, University of Houston, 4800 Calhoun Rd., N207 Engineering Bldg 1, Houston, TX 77204-4006, USA

  2. 2

    Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA

*Phone: 713 743 4623, Fax: 713 743 4623

Publication History

  1. Issue published online: 23 SEP 2009
  2. Article first published online: 12 MAR 2009
  3. Manuscript Accepted: 10 FEB 2009
  4. Manuscript Revised: 5 FEB 2009
  5. Manuscript Received: 23 DEC 2008

Funded by

  • Office of Naval Research
  • Institute of Biomedical Imaging Sciences
  • University of Houston Small Grants Program
  • National Institutes of Health. Grant Number: HL 63090

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

Get PDF (629K)

Keywords:

  • atherosclerosis;
  • optical coherence tomography;
  • permeability rate;
  • low-density lipoproteins

Abstract

Quantification of the diffusion of small molecules and large lipid transporting lipoproteins across arterial tissues could be useful in elucidating the mechanism(s) of atherosclerosis. Optical coherence tomography (OCT) was used to determine the effect of temperature on the rate of diffusion of glucose and low-density lipoproteins (LDL) in human carotid endarterectomy tissue in vitro. The permeability rate for glucose was calculated to be (3.51 ± 0.27) × 10–5 cm/s (n = 13) at 20 °C, and (3.70 ± 0.44) × 10–5 cm/s (n = 5) at 37 °C; for LDL the rate was (2.42 ± 0.33) × 10–5 cm/s (n = 5) at 20 °C and (4.77 ± 0.48) × 10–5 cm/s (n = 7) at 37 °C, where n is the number of samples. These results demonstrate that temperature does not significantly influence the permeation of small molecules (e.g. glucose), however, raising the temperature does significantly increase the permeation of LDL. These results provide new information about the capacity of an atherogenic lipoprotein to traverse the intimal layer of the artery. These results also demonstrate the potential of OCT for elucidating the dynamics of lipoprotein perfusion across the arterial wall. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Get PDF (629K)