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Quantitative cell bioimaging using gold-nanoshell conjugates and phage antibodies

  1. Vitaly A. Khanadeev1,
  2. Boris N. Khlebtsov2,
  3. Sergey A. Staroverov2,
  4. Irina V. Vidyasheva2,
  5. Alexander A. Skaptsov1,
  6. Ekaterina S. Ileneva1,
  7. Vladimir A. Bogatyrev1,2,
  8. Lev A. Dykman2,
  9. Nikolai G. Khlebtsov1,2,*

Article first published online: 1 MAR 2010

DOI: 10.1002/jbio.200900093

Issue

Journal of Biophotonics

Journal of Biophotonics

Volume 4, Issue 1-2, pages 74–83, January 2011

Additional Information

How to Cite

Khanadeev, V. A., Khlebtsov, B. N., Staroverov, S. A., Vidyasheva, I. V., Skaptsov, A. A., Ileneva, E. S., Bogatyrev, V. A., Dykman, L. A. and Khlebtsov, N. G. (2011), Quantitative cell bioimaging using gold-nanoshell conjugates and phage antibodies. J. Biophoton., 4: 74–83. doi: 10.1002/jbio.200900093

Author Information

  1. 1

    Department of Biophysics, Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov 410012, Russia

  2. 2

    Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia

*Phone: +7(8452)970403, Fax: +7(8452)970383

Publication History

  1. Issue published online: 1 MAR 2010
  2. Article first published online: 1 MAR 2010
  3. Manuscript Accepted: 11 FEB 2010
  4. Manuscript Revised: 9 FEB 2010
  5. Manuscript Received: 12 DEC 2009

Funded by

  • RFBR. Grant Numbers: 08-02-00399-a, 09-02-00496-a
  • Russian Federation Program “Development of the Scientific Potential of Higher School”. Grant Number: 2.2.1.1/2950
  • Special Program of RF “Scientific and Pedagogical Personnel of Innovative Russia”. Grant Number: 02.740.11.0484
  • President of the Russian Federation. Grant Number: MK-684.2009.2
  • Seventh Framework Program Photonics4Life

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Keywords:

  • silica/gold nanoshells;
  • plasmon resonance scattering;
  • bioimaging;
  • phage antibodies

Abstract

The authors describe a quantitative evaluation of the efficacy of cell labeling with plasmon-resonant light-scattering nanoparticles used as contrast agents for dark-field microscopy imaging. The experimental model is based on the biospecific labeling of pig embryo kidney (SPEV) cells with primary phage antibodies, followed by the dark-field microscopic visualization using conjugates of silica/gold nanoshells with secondary rabbit antiphage antibodies. To quantify nanoparticle binding, the authors introduce the labeling-efficacy factor (LEF) which is equal to the ratio of the bound-particle pixels per cell to the total number of pixels occupied by the cell. The LEF is calculated by an imaging-analysis algorithm based on the freely available ImageJ Java-based processing code. In terms of the LEF, a distinct difference was found between intact, nonspecifically labeled, and biospecifically labeled cells. (© 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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