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Optical coherence tomography and Raman spectroscopy of the ex-vivo retina

  1. Julia W. Evans1,2,3,
  2. Robert J. Zawadzki2,
  3. Rui Liu3,
  4. James W. Chan1,3,
  5. Stephen M. Lane1,3,
  6. John S. Werner2

Article first published online: 30 JUN 2009

DOI: 10.1002/jbio.200910022

Issue

Journal of Biophotonics

Journal of Biophotonics

Special Issue: Optical Coherence Tomography

Volume 2, Issue 6-7, pages 398–406, July 2009

Additional Information

How to Cite

Evans, J. W., Zawadzki, R. J., Liu, R., Chan, J. W., Lane, S. M. and Werner, J. S. (2009), Optical coherence tomography and Raman spectroscopy of the ex-vivo retina. Journal of Biophotonics, 2: 398–406. doi: 10.1002/jbio.200910022

Author Information

  1. 1

    Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore 94550, USA

  2. 2

    Vision Science and Advanced Retinal Imaging Laboratory, Department of Ophthalmology & Vision Science, University of California, Davis, Sacramento, CA 95817, USA

  3. 3

    NSF Center for Biophotonics Science and Technology, University of California, Davis, Sacramento, CA 95817, USA

Publication History

  1. Issue published online: 13 JUL 2009
  2. Article first published online: 30 JUN 2009
  3. Manuscript Accepted: 10 JUN 2009
  4. Manuscript Revised: 1 JUN 2009
  5. Manuscript Received: 16 MAR 2009

Funded by

  • National Eye Institute. Grant Number: EY 014743
  • National Institute on Aging. Grant Number: AG04058
  • National Science Foundation

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Keywords:

  • optical coherence tomography;
  • Raman spectroscopy;
  • retinal imaging

Abstract

Imaging the structure and correlating it with the biochemical content of the retina holds promise for fundamental research and for clinical applications. Optical coherence tomography (OCT) is commonly used to image the 3D structure of the retina and while the added functionality of biochemical analysis afforded by Raman scattering could provide critical molecular signatures for clinicians and researchers, there are many technical challenges to combine these imaging modalities. We describe an OCT microscope for ex-vivo imaging combined with Raman spectroscopy capable of collecting morphological and molecular information about a sample simultaneously. We present our first results and discuss the challenges to further development of this dual-mode instrument and limitations for future in-vivo retinal imaging. (© 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

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